DNA image cytometry on sections as compared with image cytometry on smears and flow cytometry in melanoma
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Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma
BACKGROUND The purpose of this study was to compare DNA histograms obtained by standard flow cytometry (FC) and high fidelity image cytometry on sections (ICS) in normal gastrointestinal mucosa and Barrett's adenocarcinoma (BAC). METHODS Archival formalin-fixed paraffin-embedded tissue blocks of 10 normal controls from 10 subjects and 42 BAC tissues from 17 patients were examined. DNA FC was ...
متن کاملDiagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology
AIMS To determine the sensitivity and specificity of flow- and image-cytometry for the detection of DNA-aneuploidy as a marker for malignant cells in effusions. METHODS 200 effusions (80 tumor cell-positive, 74 negative and 46 cytologically equivocal) were stained with DAPI-SR for DNA-flow- and with Feulgen-Pararosaniline for -image-cytometry. They were measured using a PAS-flow-cytometer and...
متن کاملFourth Updated ESACP Consensus Report on Diagnostic DNA Image Cytometry
A task force of experts in the field of diagnostic DNA image cytometry, invited by the ESACP, and further scientists or physicians revealing experience in that diagnostic procedure (names are given in Addendum A), agreed upon the following 4th updated Consensus Report on Standardised Diagnostic DNA Image Cytometry during the 7th International Congress of that society in Caen, 2001. This report ...
متن کاملEvaluation of DNA-content of Peritoneal Lavage Cells by Flow Cytometry in Patients with Gasteric Cancer
Background & Aims: Gastric cancer is the second common cancer in Iran and it has unfortunately no diagnostic signs in early stages. Considering the role of genetic and environmental factors in the development of gastric cancer, this study aimed at evaluating DNA-content of stomach cells in patients with gastric cancer in order to find a reliable index for prediction of disease prognosis and pro...
متن کاملUse of DNA image cytometry in addition to flow cytometry for the study of patients with advanced ovarian cancer.
Forty-five patients with advanced ovarian cancer were studied with both DNA flow cytometry (FCM) and automatic DNA image cytometry carried out with the Leiden Television Analysis System (Leytas). There was a significant difference in survival between the diploid and nondiploid cases as determined by FCM. Furthermore, the presence of nuclei with a high DNA content (defined as a DNA content highe...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1996
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19960901)25:1<82::aid-cyto9>3.0.co;2-g